Purchase the Reference Guide to Liquid Chromatography (LC) for Method Development titled “LC Troubleshooting: The Perfect Method by John Dolan” for $39.95 online at IndustryMatter.com. 

This reference manual is ideal for people in the liquid chromatography (LC) business and for professionals providing bioanalytical contract services to the pharmaceutical industry. Learn to solve liquid chromatography problems like leaks and pressure by using this troubleshooting guide.

Liquid chromatography (LC) is a separation technique in which the mobile phase is a liquid. Liquid chromatography can be conducted either in a column or a plane.  This guide to liquid chromatography for method development was compiled from a variety or resource materials supplied by LCGC North America magazine and Chromatographyonline.com, the premier global resource for unbiased, peer-reviewed technical information on the field of chromatography and the separation sciences. 

John Dolan has been writing "LC Troubleshooting" for LCGC for over 25 years. One of the industry's most respected professionals, John is currently a principal instructor for LC Resources, Walnut Creek, California. He is also a member of LCGC's editorial advisory board.

Nearly every chromatographer needs to do some kind of method development at one time or another. Whether your job is running a routine liquid chromatography (LC) method that needs an occasional “tweak,” you need to develop a one-use method to support chemical synthesis, or you need a robust method to monitor a production process, a good understanding of the principles of LC method development are valuable to know. John Dolan titled this series “The Perfect Method,” a little tongue-in-cheek, because, at least in his experience, there is no such thing as a “perfect” method — every method I have seen can always be made better. Herein lies the first principle of method development: “better is the enemy of good enough.” You can always make the method just a little better, but it comes at a cost of time that you might not be able to afford. Develop a method that is adequate for the job at hand, then stop.

The 7 part series includes:

• Part I: What Is Your Goal?
• Part II: Where to Start?
• Part III: Adjusting Retention
• Part IV: Controlling Peak Spacing
• Part V:  Changing Column Selectivity
• Part VI: Make It Faster
• Part VII: The Gradient Shortcut