“Column Watch,” columnist Ron Majors and guest coauthor, Prof. Peter Carr of the University of Minnesota have updated the popular HPLC-LC glossary that covers common and not-so-common terms used in conjunction with the practice of liquid phase separations.

The  Glossary of HPLC and LC Separation Terms includes common and not-so-common terms and “buzz words” for reference to high performance liquid chromatography (HPLC) columns and column technology was published (1). It is time for an update as new terms have arisen or, in some cases, their original meanings have expanded or changed. We have also decided to expand the terms dealing with HPLC and LC to cover some of the
common terms that we neglected to include in the earlier glossary. We will stick to the conventions of the International Union of Pure and Applied Chemistry (IUPAC) in their “Nomenclature for Chromatography” that provides guidance and changes in some of the more commonly accepted terms (2). Because there is still widespread usage of nomenclature that is not in alignment with the IUPAC definitions, those terms specifically recommended by them will be followed by a (IUPAC) in parentheses.

The glossary is not intended to be an in-depth listing or highly theoretical coverage. For example, we have elected not to cover the myriad of terms used in instrumentation, detection, data handling, and validation associated with HPLC analysis but have chosen to use terms that may be encountered in everyday laboratory work around columns, phases, method development, and general usage. The listing should be helpful to those just starting in HPLC but it also can serve as a refresher for long-time users in the field.

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