Conducted by John W. Dolan, Ph.D.
Author of LC/GC's popular LC Troubleshooting Column

As the most widely used analytical technique in the world, high performance liquid chromatography (HPLC) is a tool touching the lives of many laboratory workers. However, the fundamental background in separations that most of us have is based on a lecture or two during an analytical chemistry class. This Audio Seminar CD covers the basics of the HPLC process. Whether you are an experienced user that needs a tune-up or a newcomer to HPLC, this Audio Seminar CD will provide a solid foundation for understanding what is happening behind the scenes in the HPLC process.

Over the last 30 years, Dr. Dolan has designed LC equipment, developed methods, managed a contract research laboratory, taught thousands of students in LC techniques, and written over 100 papers on LC. His popular LC Troubleshooting column in LC/GC has been the go-to article each month for more than 20 years. He draws on this experience and his expertise in HPLC for this Audio Seminar CD.

This audio seminar CD will cover these specific areas:

• How does HPLC work?
• How can I tell if my separation is giving me reasonable results?
• Are there ways to quickly gauge the quality of a separation?
• What are the key measurements that I should make?
• Can I predict what will happen when I change something?

This Audio Seminar CD is presented by Dr. John W. Dolan, worldwide expert on method development and troubleshooting liquid chromatography. .

A detailed outline follows below.

Fundamentals of HPLC Separation

Part I
How does chromatography work?

• An historic perspective
• The process controlling retention
• What determines peak width
• Critical measurements and estimates

Part II
The importance of the basics

• The fundamental resolution equation
• Solvent strength and retention
• Critical factors to control selectivity
• Factors affecting peak width

Part III
Taking advantage of the relationships

• Retention vs. mobile phase concentration
• Between or beyond? - what to do with initial runs
• Easy of powerful first?
• Economics - getting the return on your investment

John W. Dolan, Ph.D.

Dr. John Dolan is most widely known as the author of LCGC’s popular LC Troubleshooting column. As editor of this column for over 20 years, Dr. Dolan has contributed more than 200 installments of practical advice to practicing chromatographers. John has written over 100 technical papers, many of which support the development and practical application of gradient elution LC. His book (with Lloyd Snyder), Troubleshooting LC Systems, is a standard reference in laboratories worldwide. He is the 2002 recipient of the prestigious Palmer Award given by the Minnesota Chromatography Forum.

Dr. Dolan managed a regional contract research laboratory for eleven years for LC Resources and later Bioanalytical Systems, Inc. The lab, which he helped found in 1988 specializes in the development, validation, and application of LC-MS/MS methods to determine pharmaceutical compounds in biological matrices. In addition, he is one of the founders of LC Resources, Inc., a company dedicated to training chromatographers around the world. As an instructor for LCR and the American Chemical Society, John has instructed more than 10,000 students in practical LC techniques.