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Common Problems with Gradient Elution Liquid Chromatography: Proven Solutions and Prevention Strategies

Item Number: LCPR100
List Price: $397.00
Price: $199.00
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Conducted by John W. Dolan, Ph.D. Author of LC/GC's popular LC Troubleshooting column

Seminar Description

Gradient elution liquid chromatography has a reputation for being a problematic procedure. As a result, many workers avoid routine gradient methods. Much of this poor reputation is a carryover from historic problems with equipment that do not apply to modern instrumentation. In fact, gradient elution is a technique that can be much more powerful than isocratic methodology whether for research purposes or routine analysis. But to obtain trouble-free methods and to have high confidence in the results, you must have a well-characterized system and understand some of the potential pitfalls of gradient methods.

This audio seminar CD is conducted by Dr. John W. Dolan. Over the last 30 years, Dr. Dolan has designed LC equipment, developed methods, managed a contract research laboratory, taught thousands of students in LC techniques, and written over 100 papers on LC. He draws on this experience and his expertise in gradient elution for this seminar. Specific areas that will be covered include:

  • How can I determine if the LC equipment is working properly?
  • What specific tests can I run to check system performance?
  • What are the causes (and fixes) for ghost peaks?
  • Why does the baseline drift in gradient runs?
  • What causes retention time variability?

This Audio Seminar CD is presented by Dr. John W. Dolan, worldwide expert on gradient elution and troubleshooting liquid chromatography. In this unique audio seminar format, lasting just one hour, Dr. Dolan uses a case-study format to illustrate the symptoms of gradient elution problems, how to fix the problems, and how to avoid them in the future.

A detailed outline follows below.

    Common Problems with Gradient Elution Liquid Chromatography: Proven Solutions and Prevention Strategies

    Part I
  • Learn how to ensure your gradient LC system is working properly
    • The influence of dwell volume
    • How to minimize gradient transfer problems
    • Measurement of dwell volume
    • How to determine gradient linearity and accuracy

    Part II
  • The cause and cure of ghost peaks
    • Running a blank gradient
    • Gradient drift and how to combat it
    • Potential sources of ghost peaks
    • Impurities from reagents and equipment
    • Tricks to exorcize ghost peaks

    Part III
  • Retention variability and how to minimize it
    • Equipment problems
    • The influence of molecular weight
    • Factors controlling retention
    • How to keep selectivity constant

John W. Dolan, Ph.D.

Dr. John Dolan is most widely known as the author of LC/GC's popular LC Troubleshooting column. As editor of this column for over 20 years, Dr. Dolan has contributed more than 200 installments of practical advice to practicing chromatographers. John has written over 100 technical papers, many of which support the development and practical application of gradient elution LC. His book (with Lloyd Snyder), Troubleshooting LC Systems, is a standard reference in laboratories worldwide. He is the 2002 recipient of the prestigious Palmer Award given by the Minnesota Chromatography Forum.

Dr. Dolan currently manages BASi Northwest Laboratory, a regional contract research laboratory of Bioanalytical Systems, Inc., that he helped found in 1988. The lab specializes in the development, validation, and application of LC-MS/MS methods to determine pharmaceutical compounds in biological matrices. In addition, he is one of the founders of LC Resources, Inc., a company dedicated to training chromatographers around the world. As an instructor for LCR and the American Chemical Society, John has instructed more than 10,000 students in practical LC techniques.